The C-terminal dsRNA-binding domain of Drosophila Dicer-2 is crucial for efficient and high-fidelity production of siRNA and loading of siRNA to Argonaute2

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FIGURE 5.
FIGURE 5.

N-terminal helicase domain, but not C-terminal dsRBD, of Dicer-2 is crucial for interaction with R2D2. HA-tagged transgenic Dicer-2 proteins in ovary lysate were immunoprecipitated by anti-HA beads and the copurified proteins were examined by Western blotting. αTubulin and Vasa served as loading controls and were not coimmunoprecipitated with HA-Dicer-2 proteins. The results were reproduced with three biologically replicated samples and representative images are shown.

This Article

  1. Published in Advance April 17, 2017, doi: 10.1261/rna.059915.116 RNA 23: 1139-1153

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