Sequences and secondary structures of all RNA constructs used in this study. (A) Human tRNALys3 and human tRNAPro. (B) MA-binding RNA aptamer (“SELEX”) and a random sequence control (“Random”). (C) HIV-1 5′ UTR with TAR/polyA, PBS, and Psi domains indicated. Psi-ΔDIS is the same as Psi RNA with the exception of the loop nucleotide replacement indicated to eliminate dimerization. To facilitate in vitro transcription, the PBS RNA contains three additional G and C nucleotides at its 5′ and 3′ termini, respectively. Psi and Psi-ΔDIS RNAs contain two additional G residues at their 5′ ends. Note that several 5′ UTR secondary structures for NL4-3 in addition to the one shown have recently been reported (Abd El-Wahab et al. 2014; Keane et al. 2015; Smyth et al. 2015). (D) Psi-SL1 and Psi-SL1,2 RNAs are truncated Psi RNA constructs also containing three extra G residues at their 5′ ends.
