Efficient in situ detection of mRNAs using the Chlorella virus DNA ligase for padlock probe ligation

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FIGURE 4.
FIGURE 4.

Comparison of the PBCV-1 DNA ligase with the T4 ligase within an in situ mRNA detection with padlock probes. The box plot shows the RCA dot count per cell for the three representative padlock probes for the ATCB mRNA and their negative controls. RCA dot counts are normalized to the result of the PBCV-1 DNA ligase.

This Article

  1. Published in Advance November 22, 2016, doi: 10.1261/rna.057836.116 RNA 23: 250-256

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