Efficient in situ detection of mRNAs using the Chlorella virus DNA ligase for padlock probe ligation

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FIGURE 3.
FIGURE 3.

Efficiency of the padlock probe assay using the PBCV-1 DNA ligase is dependent on the target position and nucleotide sequence. (A) Schematic of the ATCB mRNA sequence with indicated hybridization region of the six padlock probes (PP). The donor/acceptor nucleotide pair of each PP is given in the green filled circles. (B,C) Representative images of cells subjected to the padlock probe assay targeting the ATCB mRNA with the PP-2, 3, 4, and 6. Outlines of the cells and nucleus are obtained by image segmentation.

This Article

  1. Published in Advance November 22, 2016, doi: 10.1261/rna.057836.116 RNA 23: 250-256

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