Efficient in situ detection of mRNAs using the Chlorella virus DNA ligase for padlock probe ligation

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FIGURE 2.
FIGURE 2.

Parameter optimization of the padlock probe assay for detection of in situ mRNAs using the PBCV-1 DNA ligase. Variations in the RCA dot count per cell upon changing the padlock probe (A), RCA primer (B), PBCV-1 DNA ligase concentration (C). All other reaction parameters were left constant. Gray and white box plots denote PP-1 and PP-1n, respectively.

This Article

  1. Published in Advance November 22, 2016, doi: 10.1261/rna.057836.116 RNA 23: 250-256

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