Improvements to CE processing steps reduce data analysis errors. (A) Four primary steps are carried out during analysis of CE data; signal alignment, data preprocessing, sequence alignment, and reactivity calculations. The two main areas of improvement, signal alignment and peak picking, are highlighted in green dashed boxes. (B) Errors associated with signal alignment in QuSHAPE are eliminated using an improved alignment strategy that utilizes internal size standards. Blue and red lines indicate the minus and plus traces, respectively, (same in C). (C) The frequency of misidentified peaks in QuSHAPE is significantly reduced in RiboCAT via introduction of a peak sharpening algorithm. Dots indicate picked peaks. Orange and blue arrows denote missed or incorrectly picked peaks, respectively.
