Single-molecule FRET-Rosetta reveals RNA structural rearrangements during human telomerase catalysis

Human RNP models reveal structural homology between human and Tetrahymena RNA architectures. (A) The Tetrahymena cryoEM structure is reduced to depiction of the TERT protein component and the pseudoknot fold. (B) Comparison of P1 not formed core domain (red) model versus the Tetrahymena pseudoknot fold (opaque red overlay; distance between pseudoknot fold centers-of-mass: 1.9nm). (C) Comparison of state iii activity-based RNA architecture versus the Tetrahymena pseudoknot fold (opaque red overlay; distance between pseudoknot fold centers-of-mass: 1.5 nm).