Comparison of SHAPE reagents for mapping RNA structures inside living cells

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FIGURE 1.
FIGURE 1.

Overview of SHAPE reagents and experimental strategies. (A) Graphic representing the relative half-life and solubilities of SHAPE reagents, which are used for a variety of RNA structure probing methods, including icSHAPE and SHAPE-MaP. Actual structures are shown. (B) Schematic of various methods that take advantage of SHAPE electrophile chemistry. Key steps and differences between each method are shown in the figure.

This Article

  1. Published in Advance November 22, 2016, doi: 10.1261/rna.058784.116 RNA 23: 169-174

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