ASC1 and RPS3: new actors in 18S nonfunctional rRNA decay

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FIGURE 6.
FIGURE 6.

(A) Plasmid shuffle experiment showing growth of strains (10-fold dilution series) harboring plasmids expressing wild-type RPS3 or indicated C-terminal truncation variant on a LEU−URA− or LEU−5-FOA plate. (B) Time course analysis of 18S rRNA in rps3 yeast strains. Representative Northern blots of tagged 18S and tagged 25S rRNA and graphs summarizing multiple (n = 3) biological replicates. Normalization and error bars as in Figure 1B and 1C. (C) Multiple sequence alignment of RPS3 protein sequences.

This Article

  1. Published in Advance September 27, 2017, doi: 10.1261/rna.061671.117 RNA 23: 1946-1960

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