ASC1 and RPS3: new actors in 18S nonfunctional rRNA decay

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FIGURE 3.
FIGURE 3.

(A) Polysome profiles. (Top) Representative sucrose gradient trace for the parental strain. (Bottom) Methylene Blue stains and Northern blots of sucrose gradient fractions for the parental, asc1Δ, dom34Δ, and asc1Δ;dom34Δ strains harboring the 18S:A1492C plasmid. Boxes show positions of 40S, 60S, and 80S ribosomes. (B) Scatter plot of mean 18S:A1492C half-lives (n = 3) versus mean growth rates (doubling time; n = 3) of various yeast strains used in current study.

This Article

  1. Published in Advance September 27, 2017, doi: 10.1261/rna.061671.117 RNA 23: 1946-1960

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