Structure and RNA recognition of ribosome assembly factor Utp30

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 5.
FIGURE 5.

Heatmap of proteins in 90S preribosomes. The first sample (ITS1-239/Noc4-TAP) was purified via a plasmid-derived 5′-MS2-tagged pre-rRNA fragment ending at position 239 of ITS1 and Noc4-TAP and represents an unprocessed state of mature 90S with most labile factors released. The other samples were purified via Noc4-TAP, Utp30-TAP, and Noc4-TAP in utp30Δ strain. The first two samples from our previous study were included for comparison. Relative spectral abundance factors (RSAF) were normalized against six UTPB proteins and color-coded. The labile factors dissociated in mature 90S particles are colored magenta.

This Article

  1. Published in Advance September 26, 2017, doi: 10.1261/rna.062695.117 RNA 23: 1936-1945

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA