Sequence determinants of the folding properties of box C/D kink-turns in RNA

FRET analysis of ion-dependent folding of Kt-7 and box C/D k-turns and their variants with exchanged sequence elements. Final values of E_FRET in titrations with Mg²⁺ ions for Hm Kt-7 and Af box C/D k-turns and their variants. Species derived from Hm Kt-7 are shown in red, and those derived from Af box C/D are shown in blue. The unmodified species are shown with filled circles and all the variants with open circles. Those for which no folding was detected by FRET are drawn below the line at the base of the plot. All values of final E_FRET are given in Table 1.