Effects of miR-122 precursor miRNA on Insig1 isoform translation. Cells were transfected with YFP:Insig1 3′ NCR with precursor hairpin or duplex mimetic miR-122 or miR-141 RNA at the indicated concentrations. Shown is the fold change in YFP protein relative to miR-141-transfected cells at each concentration. (A) Diagram of reporter mRNA with marking of putative miR-122 binding sites (B) Effects of duplex mimetic miR-122. (C) Dose-responsiveness of pre-miR-122 effects on YFP:Insig1. (D) Effect of Dicer-resistant pre-miR-122 (pre-miR-122-dNx9). The data are representative of three independent replicates. Error bars represent standard error of the mean; ns, not significant; (*) P < 0.05, (**) P < 0.01, Student's t-test.
