FIGURE 1.
Expression analysis of Insig1 mRNA isoforms. (A) Diagram of Insig1 isoform 3′NCRs. The 1.4-, 1.9-, and 3-kb isoforms are revealed by Northern blot analyses (top). The locations of putative polyadenylation sites (arrows) and seed match sequences for miR-122 (tags) are indicated. The diagram shows the predicted interaction of miR-122 with the AUUAAA-polyadenylation and CA-cleavage sequence motif that generates the 1.4-kb Insig1 isoform mRNA. (B) Insig1 polyadenylation site usage in YFP-Insig1-3′NCR chimeric mRNAs. Size and integrities of chimeric mRNAs were analyzed in Northern blots using hybridization probes against YFP.
