Yeast Prp2 liberates the 5′ splice site and the branch site adenosine for catalysis of pre-mRNA splicing

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FIGURE 3.
FIGURE 3.

Analysis of tertiary RNA interactions in the catalytic core of the yeast Bact and B* spliceosomes. (A) N7-purine accessibilities of the nucleotides of the spliceosomal RNAs in the Bact and B* complexes as indicated in Figure 1C. (B,C) Representative gels of the chemical modification of the pre-mRNA around the 5′ ss (B) and of the U6 snRNA (C) in purified Bact and B* complexes (as indicated above the lanes) analyzed by primer extension. Key nucleotides are highlighted.

This Article

  1. Published in Advance September 1, 2017, doi: 10.1261/rna.063115.117 RNA 23: 1770-1779

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