Splicing regulation mediated by Acinus. (A) Summary of splicing changes following Acinus depletion, as revealed by RNA-seq analysis. The graph shows the number of events in the different splicing categories, as well as the direction of those changes. (B) Validation of alternative cassette exon for the PPHLN1 and ECT2 transcripts in Acinus-depleted cells using two distinct siRNAs. The blue arrow indicates the isoform showing an increase in Acinus-depleted cells. The proportion of transcript t1, corresponding to the longest isoform, was estimated after quantification of the transcript's abundance by an Agilent Bioanalyzer. (C) An RNA map of Acinus binding sites within regulated alternative cassette exons. Data for Acinus silenced exons (n = 30) are shown in orange, data for Acinus enhanced exons (n = 31) are in purple, and the control events (n = 185) are in gray. Acinus iCLIP common peaks were used for this analysis. The exonic normalized counts are shown as a histogram (bin interval 5 nt) for regions 50-nt downstream or upstream the splice site. The distribution of the relative distance in introns is shown as a normalized count density (dotted lines).
