The RNA-binding profile of Acinus, a peripheral component of the exon junction complex, reveals its role in splicing regulation

Acinus binds introns with weak splice sites close to the intron boundaries. (A) Histogram showing the number of Acinus common peaks in introns depending on the distance of the peak summit from and to the exon–intron junction boundary. (B) Box plot of the intron length (Log₁₀), GC content, 5′ splice strength, and 3′ splice strength for all (n = 129,270), CLIP− (n = 23,654), and CLIP+ (n = 4838) introns. Acinus CLIP+ and CLIP− introns were defined according to a read count approach. The splice strength was calculated using the maximum entropy model (Yeo and Burge 2004). The P-value evaluated with the two-sided Wilcoxon rank-sum test is indicated.