Evolving specificity of tRNA 3-methyl-cytidine-32 (m3C32) modification: a subset of tRNAsSer requires N6-isopentenylation of A37

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FIGURE 4.
FIGURE 4.

m3C32 modification in mouse and human cells. (A) Heat map of 121 unique mouse embryonic fibroblast (MEF) tRNA sequences with C at position 32, 34 of which have significant, ≥0.1, misincorporation at position 32. (B) Bar plot of the 34 tRNAs from A that have ≥0.1 misincorporation at C32. (C) Bar plot for all unique tRNAs that show significant C32 misincorporation in human A549 cells. (D) Bar plots for all unique tRNAs from MEFs and A549 cells that show significant misincorporation at C47:3. Error bars indicate standard deviation of two replicates each for MEFs and A549.

This Article

  1. Published in Advance June 27, 2016, doi: 10.1261/rna.056259.116 RNA 22: 1400-1410

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