Two sequence homologs for TRM140 in S. pombe. (A) Cartoon showing the general domain architecture of S. cerevisiae TRM140 and the two S. pombe homologs. Position of +1 frame shift in S. cerevisiae gene is indicated as +1 FS, which corresponds to amino acid 301; amino acid numbering is further indicated in panel B. (B) Alignment of the two S. pombe homologs of TRM140 with the S. cerevisiae protein. Only the C-terminal domain of the S. cerevisiae protein (starting from 301) is shown. Asterisks indicate amino acids D466, D547, and the horizontal line indicates the C-terminal 20 amino acids important for catalytic activity of TRM140 (Noma et al. 2011). (C) Phylogenetic tree of trm140+ and trm141+ homologs in different species generated from an alignment created using MUSCLE in Jalview (Edgar 2004; Waterhouse et al. 2009).
