The S. pombe tRNAs for serine and threonine have m3C32 modification. (A) A cartoon of schematized tRNA anticodon loop with different nucleotide positions indicated as referred to in the text. (B) The tRNASerUGA and tRNAThrUGU track profiles from IGV display software showing misincorporations as colored bars (Arimbasseri et al. 2015). IGV introduces color by default if ≥15% mismatch is detected relative to the reference gene sequence. Color key: green, A; red, T; orange, G; blue, C. Sequence read counts are indicated on the y-axis. (C) Plot showing C32 misincorporation levels for all S. pombe tRNAs that have C at position 32. Error bars indicate standard deviation of four replicates. The tRNAsSer and tRNAsThr are further grouped according to base identity at position 36; note that three of the four tRNAsSer contain A36 while the other tRNASer contains U36 as do all of the tRNAsThr. (D) Heat map showing fractions of each nucleotide misincorporated at C32 for the seven tRNA anticodons to the right.
