The N-terminal extension of yeast ribosomal protein L8 is involved in two major remodeling events during late nuclear stages of 60S ribosomal subunit assembly

(A) Growth on solid medium of yeast expressing wild-type or mutant forms of the r-proteins expressed from plasmids, at 30°C, 37°C, and 16°C. Empty vectors are included as negative controls for growth. (B) Growth of L7V77E, L35K103-107A and L8Δ1-52, L8Δ1-70 and L8Δ233-256 strains in liquid medium at 30°C, compared to strains expressing the corresponding wild-type proteins from the pRS315 plasmid. Cultures were diluted to ensure exponential growth. OD₆₁₀ values are plotted as log (OD_t/OD₀), where OD_t is the OD after t hours and OD0 is the starting OD. (C) Incorporation of mutant versions of the ribosomal proteins into pre-ribosomes was tested by TAP-purification of pre-ribosomes followed by Western blot assays. A MYC-tag was used for detection of r-proteins L7 and L35.