The S. pombe mRNA decapping complex recruits cofactors and an Edc1-like activator through a single dynamic surface

(A) RNA decapping experiments using a different version of S. pombe Edc1. The extracted turnover rates are indicated. (B) Relative decapping rates (see also A and D) indicate that Edc1 increases the decapping rates by a factor of 3.7. (C) Relative decapping rates for the S. cerevisiae decapping complex in the presence of increasing amounts of ScEdc1. Addition of saturating amounts of Edc1 results in a 3.4-fold increase in the turnover rates. (D) Decapping experiments using the S. pombe enzyme in the presence of Xrn1 or the Dcp2 C-terminal region show that these proteins have no effect on the in vitro decapping activity (see also B).