The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis

Total rec8⁺-transcript levels are lower in cells carrying either MTREC or EJC mutations. (A,B) Relative expression using primers within the same exon of rec8⁺ and idh2⁺ in response to deletion of EJC orthologs (A) or MTREC members (B). Expression was normalized against act1⁺, and values are reported relative to vegetative wild type (WT = 1). Asterisks denote significant difference comparing each sample with wild type within the same group (meiosis or vegetative growth), (*) P ≤ 0.05 and (**) P ≤ 0.005.