Mnh1, but not Y14 or Rnps1, is required for meiotic splicing of rec8+ transcripts. (A) The spliced rec8+ transcripts were analyzed by qRT-PCR using primers flanking the exon–exon junction of rec8+ and idh2+ in the indicated strains during vegetative growth or meiosis. Expression was normalized against act1+, and values reported are relative to vegetative wild type (WT = 1). (B) Semiquantitative RT-PCR with primers spanning two adjacent exons of rec8+ (top). The splicing efficiencies of rec8+ transcripts at the indicated strains were calculated using the splicing index. (U) Unspliced, (S) spliced. Asterisks denote significant difference comparing each sample with wild type within the same group (meiosis or vegetative growth), (*) P ≤ 0.05 and (**) P ≤ 0.005.
