FIGURE 4.
While the other EJC orthologs interact with pFal1, only Mnh1 shows a meiotic defect. (A) pFal1 interacts with Mnh1 with or without Y14. (B) pFal1 interacts with Rnps1 with or without Y14. (C) pFal1 interacts with Mnh1 with or without Rnps1. (D) pFal1 interacts with Y14. All Co-IPs were performed simultaneously using the appropriate antibodies as indicated. (*) Nonspecific binding background bands. Arrow indicates desired Y14-HA product. (E) Diploid cells with indicated genotypes were diluted 10-fold, spotted on rich medium, and grown at 30°C for 3 d. (F) Loss of either pFal1 or Mnh1 is associated with decreased sporulation efficiency, as calculated in 2C.
