FIGURE 2.
Loss of pFal1 results in defective sporulation. (A) Diploid pfal1Δ−/− cells show a growth defect similar to that in haploid pfal1Δ cells. Cells were serially diluted 10-fold, plated on rich media (YEA), and incubated at 30°C for 3 d. (B) Sporulated cells were visualized by DAPI staining and confocal microscopy. Images are presented in three classes: full tetrads (four nuclei), deformed tetrads (three or more than four nuclei), and single cells (one nucleus). Arrowheads indicate representative cells in each class. (C) Imaged cells with indicated genotypes were counted and categorized as defined in B. Bars represent the percent of each class versus the total cells.
