Luc7 mutations affect splicing of SUS1 pre-mRNA in vivo. RNA isolated from the indicated LUC7 strains was reverse transcribed with an oligo(dT) primer. cDNAs were PCR-amplified with gene-specific sense and antisense primer pairs derived from the first and last exons of the GLC7 (panel A) and SUS1 (panel B) genes. The exon–intron organization of the pre-mRNAs is shown, with exons depicted as horizontal cylinders. The PCR products were resolved by native agarose gel electrophoresis and visualized by staining with ethidium bromide. The products of PCR amplification of genomic DNA with the same primer pairs are shown in lanes labeled “DNA.” The positions and sizes (bp) of linear duplex DNA markers are indicated on the left. The RT-PCR products of unspliced, partly spliced, and fully spliced transcripts are specified on the right.
