FIGURE 2.
Deletion and alanine-scanning mutagenesis of yeast Luc7. Truncated LUC7 alleles (top panel), alanine mutants of the cysteines and histidines of the ZnF1 and ZnF2 motifs (middle panels), and additional alanine mutants in the ZnF2 domain (bottom panel) were tested for activity by plasmid shuffle. The viable FOA-resistant luc7Δ strains bearing the indicated LUC7 alleles were spot-tested for growth on YPD agar at the temperatures specified. LUC7 alleles listed at the bottom of each panel failed to complement luc7Δ in the plasmid shuffle assay and were deemed lethal.
