Nucleotide specificity of the human terminal nucleotidyltransferase Gld2 (TUT2)

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FIGURE 2.
FIGURE 2.

RNA substrate of Gld2. Gld2 was incubated with different RNA substrates and [α-P32]-ATP as indicated. Formation of α-32P labeled RNA products was monitored by electrophoretic separation and subsequent phosphorimaging. Gld2 catalyzed [α-32P]-ATP addition to RNA substrates pre-let-7a (72 nt), mature human miRNAs let-7a-5p (22 nt), miR122 (22 nt), an oligo(A) tail mimic 15A (15 nt), and total E. coli tRNA, and total human mRNA. C is no enzyme control.

This Article

  1. Published in Advance June 9, 2016, doi: 10.1261/rna.056077.116 RNA 22: 1239-1249

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