Packaging of some pre-tRNAs requires nuclear export. (A) HEK293T cells were transfected with siRNAs targeting XPOT, XPO5, or with control nontargeting (NT) RNAs. The following day, cells were transfected with pHIV-GPP and virions harvested 24–48 h afterward. RNA extracted from cells (lanes 1–4) and virus (lanes 5–8) was subjected to Northern blotting to detect the indicated RNAs. (B) The levels of 7SL RNA in virions were determined by RT-qPCR. Normalization was to HIVgRNA. Data are mean values from three biological replicates ± SEM. (C) Quantitation of A. Data are mean values from three biological replicates ± SEM. Loading was normalized to 7SL RNA. (*) P ≤ 0.05, (**) P ≤ 0.01, (***) P ≤ 0.001 (Student's t-test).
