Transcriptomic comparison of Drosophila snRNP biogenesis mutants reveals mutant-specific changes in pre-mRNA processing: implications for spinal muscular atrophy

Alternative splicing changes in Smn missense mutants. (A) Venn diagram of overlapping alternative-splicing changes in Smn missense mutants. (B) Venn diagram of the overlap in shared splicing differences in the Smn missense mutants with either snRNP-dependent changes or Smn gene-specific changes categorized by comparison of the Phax and Smn total rRNA(−) RNA-seq. (C) Browser shot examples of an Smn gene-specific change in alternative-splicing in scarface (top) or a snRNP-dependent change in CG43394 within a PAPLA1 intron on the opposite strand (bottom). Genotypes are labeled by Smn^Tg, which was expressed in the Smn^X7 null background.