FIGURE 6.
snRNP-specific protein knockdown in S2 cells. (A) qRT-PCR of untreated cells versus those treated with dsRNA for Smn, Prp6, or Prp8 mRNAs. (B) Western blot of dUTPase levels in RNAi-treated cells. Anti-SMN and anti-α-tubulin verify SMN knockdown and load, respectively. (C) qRT-PCR verification of Prp6 and Prp8 mRNA knockdowns. Levels in untreated cells were set at 100. (D) Northern blot of snRNA levels from S2 cell knockdowns, quantified in E. U6-normalized snRNA levels of WT were set at 100.
