Transcriptomic comparison of Drosophila snRNP biogenesis mutants reveals mutant-specific changes in pre-mRNA processing: implications for spinal muscular atrophy

mRNA length changes in snRNP biogenesis mutants. Pairwise comparison of significant (false discovery rate adjusted P-value <0.05) differences in mRNA length between WT and Phax^SH/SH (A); WT and Smn^X7/D (B); and WT and Ars2^C/E (C). RNA length differences were extracted from mapped RNA-seq read files by the DaPars linear regression algorithm (see text). Percentage of distal poly(A) site usage index = PDUI. (D) Venn diagram of RNA length differences in snRNP biogenesis mutants labeled as in Figure 2.