Defects in THO/TREX-2 function cause accumulation of novel cytoplasmic mRNP granules that can be cleared by autophagy

THO and TREX-2 mutants do not show gross defects in translational repression or translational re-entry following stress. (A) WT BY4741 yeast, hpr1Δ, mft1Δ, tho2Δ (THO), and sac3Δ (TREX-2) strains were examined via polysome analysis in mid-log, during NaN₃ stress (15 min 0.5% w/v), and following 30 min of recovery (media replacement). Data representative of observations in three biological replicates (separate culture and transformant). (B) Quantitation of the NaN₃ polysome: 80S ratio before, during, and after stress. Mean ± SD shown. (C) WT BY4741 yeast, hpr1Δ and tho2Δ strains were examined as above, but following 15 min of glucose deprivation, and following 15 min of recovery. Data representative of observations in three biological replicates (separate culture and transformant). (D) Quantitation of the -Glucose polysome: 80S ratio before, during, and after stress. Mean ± SD shown. Additional glucose deprivation traces are present in Supplemental Figure 4.