Defects in THO/TREX-2 function cause accumulation of novel cytoplasmic mRNP granules that can be cleared by autophagy

Constitutive Pab1 foci in THO and TREX-2 deletion strains exhibit compositional similarities to stress granules. (A) Strains were transformed with pRP2132 (Ded1-mCh) and pRP2478 (Pab1-GFP) and examined in mid-log growth; WT strains were subject to 30 min of 0.5% w/v NaN₃ stress to induce stress granules as a positive control. Data representative of observations in two biological replicates (separate culture and transformant). (B) Strains were transformed with pRP1920 (Pbp1-mCh) and pRP2478 (Pab1-GFP) and examined as in A. Data representative of observations in two biological replicate experiments. Scale bar = 2.5 µM. Colocalization frequencies summarized in Supplemental Table S2.