The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNA^Tyr

Effects of mature tRNA^Tyr expression on the TbTrl1-deficient procyclic form T. brucei cells. (A) Northern blot analysis of 4 d RNAi induced (+tet) and uninduced TbTrl1 RNAi cells expressing recombinant mature tRNA^Tyr. Specific probes were used for Intron-containing (Intron probe) and mature (3′ exon Tyr probe) tRNA^Tyr. The position of the Intron-containing and mature tRNA^Tyr signals are indicated on the left. (B) Growth curve of uninduced (−tet, black circles) and induced (+tet, black squares) TbTrl1 RNAi expressing mature tRNA^Tyr. The y-axis shows a log-scale of cumulative cell densities accounting for total dilutions and the x-axis shows the progression of the growth curve in days.