The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNATyr

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 3.
FIGURE 3.

FACS analysis of the TbTrl1-deficient procyclic form T. brucei cells. Samples of procyclic form T. brucei cells harboring the TbTrl1 RNAi plasmid construct were taken at various times for FACS analysis following tetracycline induction. (A) Histograms from FACScan of induced (+tet) and uninduced cells (−tet). (B) Percentages of induced (+tet) and uninduced cells (−tet) in G1, S, and G2/M phases as determined by the WinMDI software.

This Article

  1. RNA 22: 1190-1199