FIGURE 1.
Schematic representation of Trl1 sequences. Trl1 sequences from Saccharomyces cerevisiae (827 aminoacids), Trypanosoma brucei (870 amino acids), and Arabidopsis thaliana (1104 amino acids) were analyzed. The gray area shows conserved regions and motifs between the sequences. Motifs Lig, I, Ia, III, IIIa, IV, and V are present in the ligase domain. Motifs GxGKT, RxxxR, and Pnk are conserved in the kinase domain. Motif IIIa and Pnk are observed only in plants. The CPD domain is marked by the presence of two HФTФ motifs. All the essential amino acid residues in yeast Trl1 ligase are underlined.
