Some HSUR1 mutants bind miR-27a without effectively triggering its degradation. (A) Coimmunoprecipitation of miR-27a from BJAB cells stably expressing either EV or various HSUR1 mutants (expressed at levels shown in Fig. 3B) with anti-Sm antibody (αSm). I, input, 1%; S, supernatant, 1%; P, pellet, 100%. Northern blots with probes for miRNAs, HSUR1 and U2 for the precipitation control. (B) Levels of HSUR1-bound miR-27a in BJAB cells expressing the HSUR1 mutants were normalized to the input. Averages from four independent experiments with standard deviations are shown. P-values between WT HSUR1 and its mutants were calculated using Student's t-test; (*) P-values <0.05.
