FIGURE 2.
Most large subunit ES are necessary for optimal growth. Serial dilutions of log phase yeast cultures were spotted onto solid medium and grown either at the permissive temperature (25°C, left panels) or at the restrictive temperature (37°C, right panels). Empty vector (“No rDNA”) and wild-type (WT) controls (“WT rDNA”) are indicated. The nonessential ES are highlighted with gray labels. Note that a less dense culture of ES9L was used. Similar results were observed across at least three independent biological replicates.
