Induced formation of Ψ93 (as well as Ψ91) under different nutrient-deprivation conditions. Log-phase cells were either kept at log phase (lanes 1 and 2), grown to saturation (lanes 7 and 8), transferred to synthetic medium lacking glucose and incubated for another hour (lanes 3 and 4), or transferred to pure water and incubated for another hour (lanes 5 and 6). Cells were then collected and cellular RNAs recovered. Pseudouridylation assay (CMC-modification followed by primer extension) was subsequently performed. Signals corresponding to the three constitutively formed pseudouridines (Ψ35, Ψ42, and Ψ44) as well as inducibly formed Ψ93 and Ψ91 are indicated by arrows.
