A whole-mount in situ hybridization method for microRNA detection in Caenorhabditis elegans

WISH analysis for detection of mir-48 miRNA in temperature-sensitive pash-1 mutants at the L4 stage. Genotypes are pash-1 (A,B) and wild-type (C). Animals were grown under restrictive conditions (A,C) and under permissive conditions (B). Images on the right in A show magnified views of the boxed regions in the image on the left, and the arrows indicate nuclei of the vulva (top) and the rectum (bottom). Scale bars, 100 µm (A–C) and 30 µm (magnified images in A).