Model of in vivo FBF-mediated RNA regulation. FBF associates with RNA motifs to repress its targets (especially transcripts that encode cell cycle regulators as depicted here), and thereby promote GSC self-renewal. Our work expands the repertoire of FBF binding (shown with relative peak heights) to locations other than the 3′UTR, to lincRNAs, and to motifs other than the canonical FBE. Enriched crosslinking regions (orange marks) indicate regions of close protein–RNA interaction in vivo. Our model depicts multiple FBFs simultaneously bound on a single RNA, but a caveat from our data is that multiple peaks may represent a population of single binding events.
