Transcription of lncRNA prt, clustered prt RNA sites for Mmi1 binding, and RNA polymerase II CTD phospho-sites govern the repression of pho1 gene expression under phosphate-replete conditions in fission yeast

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FIGURE 7.
FIGURE 7.

Effect of DSR mutation on prt and pho1 expression in rpb1-CTD-WT and -S7A cells. pho1Δ rpb1-CTD-WT and pho1Δ rpb1-CTD-S7A strains were transformed with prt–pho1 reporter plasmids (kanMX) with wild-type or mut1 + mut2 DSRs. (A) Acid phosphatase activity. (B) RT-qPCR analysis of the prt transcript. The prt transcript levels are normalized to that of rpb1-CTD-WT cells with the wild-type DSR reporter (defined as 1.0). Error bars indicate SEM.

This Article

  1. Published in Advance May 10, 2016, doi: 10.1261/rna.056515.116 RNA 22: 1011-1025

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