Transcription of lncRNA prt, clustered prt RNA sites for Mmi1 binding, and RNA polymerase II CTD phospho-sites govern the repression of pho1 gene expression under phosphate-replete conditions in fission yeast

prt promoter deletion derepresses pho1. (A) The indicated prt promoter truncations were introduced into the tandem prt–pho1 cassette depicted in the cartoon. The graph shows the acid phosphatase activity of pho1Δ cells bearing the indicated reporter plasmids (kanMX). (B) prt promoters with the indicated mutations (mut) of the HomolD and TATA elements were introduced into the −633 prt promoter of the tandem prt–pho1 cassette. The left panel shows the acid phosphatase activity of pho1Δ cells bearing reporter plasmids with wild-type (WT) or mutated prt promoter elements as specified. The right panel shows RT-qPCR analysis of the prt transcript levels; values are normalized to that of cells with the wild-type prt promoter (defined as 1.0). Error bars indicate SEM.