Transcription of lncRNA prt, clustered prt RNA sites for Mmi1 binding, and RNA polymerase II CTD phospho-sites govern the repression of pho1 gene expression under phosphate-replete conditions in fission yeast

Effect of rpb1-CTD T4A and S7A mutations on prt and pho1 promoter activities. S. pombe pho1Δ rpb1-CTD-WT, rpb1-CTD-T4A, and rpb1-CTD-S7A strains were transformed with reporter plasmids (LEU2) in which pho1 expression was driven by the prt promoter (top panels A and B) or the pho1 promoter (bottom panels C and D). Acid phosphatase activity is shown in A and C. RT-qPCR analysis of the pho1 transcript is shown in B and D. The pho1 transcript levels in T4A and S7A cells are normalized to those of rpb1-CTD-WT cells (defined as 1.0). Error bars indicate SEM.