Transcription of lncRNA prt, clustered prt RNA sites for Mmi1 binding, and RNA polymerase II CTD phospho-sites govern the repression of pho1 gene expression under phosphate-replete conditions in fission yeast

Demarcation of the pho1 promoter. Plasmid reporters of promoter activity contained 283, 219, 189, 169, 113, and 58 nt of genomic DNA upstream of the pho1 transcription start site. (A) Acid phosphatase activity of pho1Δ cells bearing the indicated pho1 reporter plasmids (LEU2). (B) RT-qPCR analysis of the pho1 transcript was performed with total RNA isolated from pho1Δ cells bearing the indicated reporter plasmids. The pho1 transcript levels are normalized to that of the −283 reporter (defined as 1.0). Error bars indicate SEM.