circTAIL-seq, a targeted method for deep analysis of RNA 3′ tails, reveals transcript-specific differences by multiple metrics

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FIGURE 2.
FIGURE 2.

(A) Density curves comparing lengths of tail populations from CO1 or CO3p transcripts. CO1 tails >100 nt are present but are not abundant enough to be observed on a chart of this scale. (B) Enrichment analysis of tail-less reads in five roughly equally populated bins. The figure is pseudo-colored, showing only significant enrichments (P-values <0.01 and fold-enrichment >1.5), with blue and yellow colors indicating underrepresentation and overrepresentation of tail-less reads, respectively. (C) Density curves comparing fraction nucleotides that are “A” in each tail [0 = tails that are oligo(U), 1 = tails that are oligo(A)] from CO1 and CO3p transcripts. Distributions were inferred using R statistical package. Area under each curve is 1. “r1” and “r2” are biological replicates.

This Article

  1. RNA 22: 477-486