FIGURE 6.
Effect of transfection of tRNAGlu isodecoder transcripts on mistranslation. In vitro transcribed tRNAGlus were transfected into stable GAA/GAG mutant cells for 36 h, followed by flow cytometry to measure mistranslation. Final values were calculated relatively to mock-transfected cells (only lipofectamine). (A) GAA stable lines. (B) GAG stable lines. Histograms represent the distribution of mCherry fluorescence intensity (average of at least three experimental replicates from two separate experiments).
