FIGURE 2.
Experimental setup to quantify Met mistranslation in live cells using dual fluorescence reporters. (A) Schematic description of strategy used for creating the stable cell lines. (B) FACS plot for HEK293 cells stably transfected with the WT plasmid. (C) FACS plot of the cells stably transfected with GAA (Glu), GAG (Glu), AAG (Lys), and GAC (Asp) mutant plasmid. The quadrants in red correspond to the strongly GFP+/mCherry+ cell population. The percentage of cells in this population is also indicated. Quadrant gates were drawn based on fluorescence emission of the WT (positive control) cell population. Debris and doublets were gated out of the analysis.
